There's no limit, and affiliates should appear in your Seller Admin > View/List Affiliates as soon as they join. Note that Date of Registration shows the date they joined E-junkie, which may be older than when they joined your particular affiliate program.
Normally a new affiliate would click your sign-up link, log into their existing E-junkie account or register a new one, and then their account should be enrolled in your program.
Sometimes if they register a new account after clicking your sign-up link, the new account may not get added to your program automatically, so in that case they just need to click your sign-up link again and log into the E-junkie account they already registered, and that should do the trick.